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The Journal of Heredity 1996:87(3):224-232
© 1996 The American Genetic Association 87:224-232

research-article

Chromosome Extraction and Revision of Linkage Group 2 in Tribolium castaneum

R. W. Beeman, J. J. Stuart, M. S. Haas, and K. S. Friesen

From the U.S. Grain Marketing Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture 1515 College Ave., Manhattan, KS 66502
The Department of Entomology, Purdue University West LaFayette, Indiana
The Division of Biology, Kansas State University Manhattan

Corresponding Editor: Therese Ann Markow

Abstract

We used a balancer chromosome to recover ethylmethanesulfonate-induced recessive mutations in a targeted region of the genome of the red flour beetle (Tribolium castaneum) by the technique of chromosome extraction. The experiments reported herein constitute the first successful application of this powerful technique in the order Coleoptera. Using the balancer chromosome maxillopedia-Dachs3 (mxpDch-3), we recovered seven recessive visible variants representing seven distinct loci and several dozen recessive lethal variants representing at least five distinct loci after screening 1,607 EMS-mutagenized chromosomes. A subset of the mxpDch-3-extracted mutations were positioned on the map of the second linkage group by a series of two-, three-, and four-point crosses. The orientation of the homeotic gene complex (HOM-C) on this linkage group was also determined. With the advent of better and more varied balancer chromosomes and the concomitant improvement of chromosome extraction procedures for genetic analysis of T. castaneum, saturation mutagenesis of targeted regions of the genome is now feasible in this species.


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